Miniprotein-Based Artificial Retroaldolase

An artificial retroaldolase was developed using a 43-amino-acid-long miniprotein derived from the C-terminal domain of MvaT protein. A series rounds rational optimization activity performed, included grafting active motif in cleft miniprotein, engineering site structure, and modifying charge distribution on surface miniprotein. It proved that miniproteins this size can be mutated to obtain an effective enzyme-like catalyst. Moreover, possibility tuning catalyst efficiency by shown.